![]() ![]() Genetic drift and transmission bottleneck of HBV quasispecies in MTCT. ![]() We also demonstrate that the data can be potentially useful to estimate the bottleneck effect during HBV MTCT, which provides information to optimize treatment for reducing the frequency of MTCT. In all four pairs of MTCT samples, we consistently observed a significant overlap of viral haplotypes shared between mother and child. Dominance of "a determinant" polymorphisms were observed in two children, which pre-existed as minor quasispecies in maternal samples. The detection limit of minor viral haplotypes reached 0.1% for individual patient sample. By assigning unique tags to individual viral sequences, we accurately reconstructed HBV haplotypes in a region of 836 bp, which contains the major immune epitopes and drug resistance mutations. Here we adopted a newly developed tag linkage deep sequencing method and analyzed the quasispecies of four MTCT pairs that broke through immunoprophylaxis. Little is known about the bottleneck of HBV transmission and the evolution of viral quasispecies in the context of MTCT. full immunoprophylaxis, mother-to-child transmission (MTCT) of Hepatitis B Virus still occurs in approximately 2-5% of HBsAg positive mothers. 18 Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, 90095, USA. ![]() 17 Cancer Institute, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Zhejiang University, Hangzhou, 310058, China. 16 Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA. 15 Key Laboratory of Infectious Disease, Laboratory of Molecular Virology, Changchun, 130021, China. 14 Key laboratory of Zoonosis Research, Ministry Education, Jilin University, Changchun, 130062, China. 13 Hepatology, The 1st hospital of Jilin University, Changchun, 130031, China. ![]() 12 Department of Medicine, Division of Infectious Diseases, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.11 Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.10 Molecular Biology Institute, University of California, Los Angeles, CA, 90095, USA.9 Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, 90095, USA.8 Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.7 Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.6 Epidemiology, The 1st hospital of Jilin University, Changchun, 130031, China.5 Key Laboratory of Infectious Disease, Laboratory of Molecular Virology, Changchun, 130021, China.4 Key laboratory of Zoonosis Research, Ministry Education, Jilin University, Changchun, 130062, China.3 Hepatology, The 1st hospital of Jilin University, Changchun, 130031, China.2 Cancer Institute, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Zhejiang University, Hangzhou, 310058, China.1 Department of Molecular and Medical Pharmacology, University of California, Los Angeles, CA, 90095, USA. ![]()
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